John Libbey Eurotext

Environnement, Risques & Santé


Toxicological response of a lung organotypic culture exposed to gasoline engine emissions Volume 7, issue 2, Mars-Avril 2008


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Institut national de la santé et de la recherche médicale (Inserm), U 644, Faculté de médecine et pharmacie de Rouen, Bd Gambetta, 76183 Rouen cedex, Laboratoire de toxicologie et hydrologie, Faculté de médecine, de pharmacie et d’odontostomatologie, Université Cheikh Anta Diop de Dakar (Ucad), BP 5005, Dakar Sénégal, Centre d’études et de recherche en aérothermie moteur (Certam), Technopôle du Madrillet, 1, rue Joseph Fourier, 76800 Saint-Étienne du Rouvray France

The study of combustion engine exhaust presents several problems, including sampling and the exposure procedure. Most studies have been conducted on diesel engine emissions, with particles collected in advance and placed in suspension in a liquid before in vivo or in vitro exposure. It is also important, however, to study gasoline engine emissions, which contain not only gaseous pollutants (CO, VOCs, and NOx) but also very small particles (30-50 nm) with high pulmonary penetration capacity. The aim of this work was to study the toxicity of the emissions of a gasoline engine and assess the impact of a pollution reduction system. A direct exposure system made it possible to expose organotypic cultures of rat lung slices to continuous exhaust flows at the engine outlet. Biological markers of the lung tissue reaction to the air contaminants were measured. Results showed that gasoline exhaust was associated with alterations in the metabolism of glutathione and energy, with decreased levels of ATP, GSH, GPx and GRED. Treatment of these emissions by a 3-way catalyst eliminated the noxious effects to pulmonary tissue that were observed without it and showed the effectiveness of this depollution system on the variables studied.