Figures
Figure 1
The effect of epidermal peeling on hair regeneration. A , B ) Epidermal peelings were conducted on the back of eight-week-old male C57BL/6 mice using a dermatome, within a rectangular area (3 cm by 1 cm). Depth 1 (∼ 0.018 mm) was strictly limited within the epidermal layer; Depth 2 (∼ 0.047 mm) was extended a little to the dermis to ensure peeling of the entire epidermis; Depth 3 (∼ 0.084 mm) was extended more to the dermis. C ) Epidermal peelings repeated at Depth 2. D ) Post-peeling log2 fold change of mRNA expression of IL-1β and TNF-α at a series of time points. Data are presented as mean ± SEM (n =3); one-way ANOVA, corrected for multiple comparisons using Dunnett test (the data at 0 hours served as the control); * p <0.05; ns, not significant. E ) Split-thickness (∼ 0.25 mm) skin peeling from the right frontal-temporal corner of the scalp using a dermatome. F ) Photographs of the scalp and macro-photographs of the peeled corner at the beginning and end of follow-up (three months), with anagen and telogen hair counts. Green or yellow circles indicate anagen or telogen hair, respectively (three days before photographs were taken, the hair was shaved to minimal length [not more than approximately 0.1 mm]). The anagen hair continued to grow and elongate to 0.9-1.2 mm, approximately three days after, while the telogen hair remained at original length.
Figure 1
Authors
1 Department of Dermatology, Xiangya Hospital,
2 Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province,
3 Center for Molecular Medicine, Xiangya Hospital,
4 National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China
Hair regeneration occurs during the cyclical telogen-anagen transition of hair follicles. In a specific region on the scalp affected by androgen alopecia (AGA), the telogen-anagen transition fails to complete, resulting in blocked hair regeneration and a specific baldness pattern [1]. A large open full-thickness wound of skin has been shown to induce hair follicle regeneration in adult mice [2, 3]. This phenomenon was later called “wound-induced hair follicle neogenesis” (WIHN) [4]. However, this [...]