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In vitro evaluation of the genotoxicity of polycyclic aromatic hydrocarbons as pure compounds and in mixtures Volume 13, issue 4, Juillet-Août 2014

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Tables

Authors
1 Univ. Grenoble Alpes
INAC, LCIB, LAN
17 rue des Martyrs
38054 Grenoble cedex 9
France
2 CEA, INAC, SCIB, LAN
17 rue des Martyrs
38054 Grenoble cedex 9
France
3 Université Joseph Fourier
UFR de médecine
CHU de Grenoble
Laboratoire TIMC (UMR CNRS 5525)
Équipe EPSP Environnement et Prédiction de la Santé des Populations
Domaine de la Merci
3870 La Tronche
France
* Tirés à part
  • Key words: air pollution, DNA damage, genotoxic stress, in vitro, polycyclic aromatic hydrocarbons
  • DOI : 10.1684/ers.2014.0710
  • Page(s) : 318-24
  • Published in: 2014

Polycyclic aromatic hydrocarbons (PAHs) are pollutants found in urban air and in many industries; several have carcinogenic properties. We studied their genotoxic potential in vitro, in particular, oxidative damage and DNA adducts. The dose-response curve for adduct formation following exposure to benzo[a]pyrene (B[a]P) depends strongly on the type of cell: it is linear in HepG2 hepatocytes, bell-shaped with a maximum at low concentrations in A549 lung cells, and flat in T24 bladder cells. The genotoxicity of simple synthetic mixtures or extracts from atmospheric samples is characterized by both inhibition and potentiation effects. Thus, at the same B[a]P concentration, the number of adducts produced differs between PAH-rich industrial samples and urban samples containing many additional types of compounds. The level of oxidative damage is much higher in the latter. These observations highlight the difficulty of risk assessment associated with exposure to PAH mixtures.