John Libbey Eurotext

Environnement, Risques & Santé


Application of in vitro models for studying pharmaceuticals hazard in fish Volume 5, issue 4, Juillet-Août 2006

Institut national de l’environnement industriel et des risques (Ineris), Unité d’évaluation des risques écotoxicologiques, BP 2, 60550 Verneuil-en-Halatte, France, Département Sciences de l’environnement et santé publique, Unité mixte de recherche (UMR) Centre national de la recherche scientifique (CNRS) 5569, Faculté de pharmacie, Avenue Charles Flahault, BP 14491, 34093 Montpellier cedex 5, France

Recent studies report the presence of pharmaceuticals and their bioactive metabolites in surface waters and raise the question of the potential ecotoxicity of these biologically active substances, especially in fish. Because of the large number of substances found in the aquatic environment, the metabolic and physiological specificities of different fish species, and the lack of data about drug toxicity in fish, in vitro testing with fish cells is currently promoted for “ethical” screening of active pharmaceutical substances. Herein, we propose an overview of the use of these fish cell models through the example of recent studies reporting the application of primary cells and cell lines (genetically modified or not) for the assessment of basal cytotoxicity and toxicity mechanisms of pharmaceuticals.