John Libbey Eurotext

European Journal of Dermatology


Induction of sister chromatid exchanges by modulated, low frequency electric fields in fibroblasts from normal donors and from patients with xeroderma pigmentosum and dysplastic nevus syndrome Volume 7, issue 2, March 1997


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Hautklinik am Klinikum Mannheim, Postfach 10 00 23, D-68135 Mannheim, Germany.
  • Key words: dysplastic nevus syndrome (DNS), dermal fibroblasts, low frequency electric fields, sister chromatid exchanges (SCE), xeroderma pigmentosum (XP).
  • Page(s) : 85-8
  • Published in: 2000

The following paper summarizes experiments carried out to find the effects of electric fields which are used in therapeutic applications (e.g. muscle stimulation, analgesia etc.). Human fibroblasts derived from normal donors, as well as from patients with xeroderma pigmentosum (XP) or dysplastic nevus syndrome (DNS) were exposed, in vitro, to weak electric 4,000 Hz fields, amplitude modulated 25 and 50 Hz, respectively. The treatment increased the rate of sister chromatid exchanges (SCE) to above the spontaneous level in all fibroblast types. The maximum effect was found for XP-cells at a field strength of 1 V/m (50 Hz). However the increase of SCE was not high as in comparable experiments with nocives, e.g. UV-light. A large increase (4 times higher) in field intensity did not result in higher SCE rates and even lowered the response at 50 Hz. No differences were observed between the different types with the exception of a slightly higher responsiveness of the XP-fibroblasts at the lower field intensity. Variance analysis revealed statistically significant relationships for the dependence of SCE-induction upon field amplitude and for the interaction between amplitude and modulation frequency