John Libbey Eurotext

Virologie

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Apport de l’immunocapture-Elisa au diagnostic de l’infection à virus grippal A(H1N1)pdm09 : une alternative à la RT-PCR ? Volume 15, issue 3, Mai 6

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Authors
HIA Legouest, Laboratoire de biologie médicale, 27, avenue de Plantières, BP 90001 57077 Metz Cedex 3, France, Groupement hospitalier Est, Institut de microbiologie CNR du virus influenza pour la région Sud, 59, boulevard Pinel, 69677 Bron Cedex, France

Aim of the study. To evaluate immunocapture-Elisa (ICE) sensitivity versus RT-PCR in diagnosis of influenza A(H1N1)pdm09 virus infection. Methods. Sixty-seven RT-PCR-negative and 282 RT-PCR-positive nasopharyngeal swabs collected during winter 2009-2010 have been analyzed using ICE. Results. Among all the samples tested, a sensitivity of 31.3% was found for ICE. The sensitivity of ICE was directly correlated to the virus load determined through the number of cycling reactions necessary to reach detection by RT-PCR. Conclusion. ICE can be a suitable method compared to RT-PCR when RT-PCR cannot be used for economical or epidemiological reasons. Its sensitivity is largely dependent of the nasopharyngeal sampling quality.