Medical College, Wuhan University of Science and Technology, Wuhan, Hubei Province, P.R. China
Department of Basic Medical Laboratory, General Hospital of Central Theater Command, Wuhan, Hubei Province, P.R. China
Department of Laboratory Medicine, Wuhan Hanyang Hospital, Wuhan, Hubei Province, P.R. China
Correspondence: B. Diao, Department of Basic Medical Laboratory, General Hospital of Central Theater Command, Wuhan, Hubei Province, P.R. China.
The authors contributed to the work equally.
Magnesium transporter 1 (MAGT1) is a key protein that regulates the level of free Mg2+ in cells. Previous studies found that the downregulation of MAGT1 expression in CD8+T cells of HBV patients was correlated with the decrease of intracellular magnesium. However, the expression of MAGT1 mRNA in the CD8+T cells from HBV patients was not significantly altered, indicating that the change in MAGT1 expression was accomplished through posttranscriptional regulation. Through bioinformatics and qRT-PCR detection, miR-199a-5p was found to have a target gene relationship with MAGT1. The expression levels of miR-199a-5p and MAGT1 in HBV infection were evaluated. Lentivirus assays were used to analyze the effects of miR-199a-5p upregulation and downregulation on the MAGT1 expression level and the immune system. Results showed no significant change in the expression of MAGT1 mRNA in HBV-infected cell lines, but the expression of MAGT1 was downregulated. Additionally, the expression level of miR-199a-5p was significantly increased. To this end, we predicted a target relationship between miR-199a-5p and MAGT1 by using TargetScan and verified this relationship through a luciferase activity reporter gene assay. As a result, MAGT1 was found to be the direct target of miR-199a-5p. The targeted inhibition of MAGT1 induced by miR-199a-5p overexpression led to the immune function depletion of CD8+T cells in HBV patients. Downregulating the expression level of miR-199a-5p could effectively improve the functional depletion of CD8+T cells. These findings indicate that miR-199a-5p and MAGT1 could potentially be used as biomarkers for the diagnosis and treatment of chronic HBV infection.