European Journal of Dermatology
MENUPCR-based diagnosis of Sporothrix infection using DNA from paraffin-embedded skin specimens in previously undiagnosed cases Volume 30, numéro 5, September-October 2020
Illustrations
1 Department of Dermatology, Dokkyo Medical University
2 Division of Clinical Science, Dokkyo Medical University, School of Medicine
- DOI : 10.1684/ejd.2020.3862
- Page(s) : 614-5
- Année de parution : 2020
Definitive diagnosis of dermatomycosis requires identification based on culture of focal eruption samples. However, it is occasionally necessary to also consider culture-negative cases and cases without culture. PCR may aid in the diagnosis of such cases. However, although nested-PCR (involving two rounds of PCR amplification) is highly sensitive, it is liable to contamination, which may affect the interpretation of results [1].We previouslyreported identification of Sporothrix based on extraction [...]