John Libbey Eurotext

European Journal of Dermatology


PCR-based diagnosis of Sporothrix infection using DNA from paraffin-embedded skin specimens in previously undiagnosed cases Volume 30, numéro 5, September-October 2020


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1 Department of Dermatology, Dokkyo Medical University
2 Division of Clinical Science, Dokkyo Medical University, School of Medicine

Definitive diagnosis of dermatomycosis requires identification based on culture of focal eruption samples. However, it is occasionally necessary to also consider culture-negative cases and cases without culture. PCR may aid in the diagnosis of such cases. However, although nested-PCR (involving two rounds of PCR amplification) is highly sensitive, it is liable to contamination, which may affect the interpretation of results [1].We previouslyreported identification of Sporothrix based on extraction [...]