John Libbey Eurotext

Magnesium Research

Rumen epithelial cells adapt magnesium transport to high and low extracellular magnesium conditions Volume 22, numéro 3, september 2009

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  • Auteur(s) : Monika Schweigel, Judith Kuzinski, Carolin Deiner, Martin Kolisek , Research Unit Nutritional Physiology “Oskar Kellner”, Research Institute for the Biology of Farm Animals (FBN), Dummerstorf, Department of Veterinary Physiology, Free University of Berlin, Berlin, Germany
  • Mots-clés : sheep, rumen, Na <sup>+</sup>/Mg <sup>2+</sup> exchanger, TRPM7, MagT1, mag-fura 2
  • Page(s) : 133-50
  • DOI : 10.1684/mrh.2009.0176
  • Année de parution : 2009

A protein of ~ 70-kDa was identified as a candidate Na +/Mg 2+ exchanger in rumen epithelial cells (REC). Melastatin-related Transient Receptor Potential 7 (TRPM7) and Magnesium Transporter 1 (MagT1) transcripts and, from them, encoded proteins were also detected. The regulation of these Mg transport pathways by extracellular [Mg] changes was the main focus of this study. Therefore, a 24-h pre-incubation of ovine REC in control (1.2 mM), low (0.12 mM)-Mg, and high (5 mM)-Mg medium was performed. Na +/Mg 2+ exchangers, TRPM7 and MagT1 abundance and activity were investigated by Western blot analysis, flow cytometry, immunocytochemistry and fluorescence spectroscopic measurements of [Mg 2+] i changes. Inhibitors were employed to differentiate Na +/Mg 2+ exchanger-mediated (imipramine) and channel-mediated (cobalt(III)hexaammine, nitrendipine) Mg transport. Basal [Mg 2+] i (0.40 ± 0.02 mM) was not influenced by pre-incubation in low- or high-Mg medium. However, compared with control REC (4.1 ± 0.7 μM/min), such cells showed reduced (2.8 ± 0.6 μM/min) or elevated (6.4 ± 0.9 μM/min) Mg extrusion rates that correlated with a decreased (25%) and increased (38%) expression of the putative Na +/Mg 2+ exchanger protein, respectively. Low- and high-Mg pre-incubated REC were both characterized by an increased (30-40%) influx capacity. In low-Mg REC, the latter resulted mainly from a strong activation of the TRPM7-related transport component. The data thus clearly demonstrate the intrinsic regulation of REC transmembrane Mg transport.