Institute of Immunology, University of Rostock, Schillingallee 70, 18055 Rostock, Hans Knoell Institute for Natural Products Research, Beutenbergstr. 11a, 07745 Jena, Institute for Medical Informatics and Biometry, University of Rostock, Rembrandtstr. 16-17, 18057 Rostock, Department of Dermatology and Venereology, University of Rostock, Augustenstr. 80-84, 18055 Rostock, Germany.
- Mots-clés : ANXA3, annexin A3, COX-2, cyclooxygenase-2, CDKN1C, cell cycle dependent kinase inhibitor 1C, DSC2, desmocollin-2, IL-8, interleukin-8, PBEF, pre-B cell enhancing factor
- Page(s) : 251-7
- Année de parution : 2005
In the present report gene expression profiling of peripheral blood mononuclear cells (PBMC) from psoriasis patients suffering from severe generalized disease was performed comparing diseased stage with cured stage. By this means, 18 genes were identified which showed differential expression. The most significant differences were found for IL-8, annexin A3, cycloxygenase-2 (COX-2), cell cycle regulator G0S2, and pre-B cell enhancing factor (PBEF), all of which showed upregulation in the diseased stage. Microarray data were confirmed by real-time RT-PCR. Further analyses using support vector machines identified three pairs of genes (IL-8 – CDKN1C/p57, cyclooxygenase-2 – NR1D2, and desmocollin-2 – CDKN1C/p57) which allowed an accuracy of disease stage prediction of 86%, based on gene expression patterns. Taken together, this is the first large-scale gene expression study of psoriasis PBMC identifying candidate genes that might contribute to psoriasis immunopathogenesis. The genes identified in the present report and the molecular mechanisms underlying their regulation might serve as future targets for therapeutic intervention in psoriasis.