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European Journal of Dermatology

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Development and in vitro assay of oxidative stress modifying formulations for wound healing promotion Volume 21, supplément 2, May 2011

Auteurs
University of Lyon, EA4169 Physiological and Pathological Functions of the Cutaneous Barrier, Laboratory for R&D in Industrial Pharmaceutical Technologies, 8, Av Rockefeller, F-69373 Lyon cedex 08, University of Lyon, EA4169 Physiological and Pathological Functions of the Cutaneous Barrier, Laboratory for Dermatological Research, E. Herriot Hospital, wing R, Place d’Arsonval, F-69437 Lyon Cedex 03, Laboratoire dermatologique Bioderma, 75, cours Albert Thomas, F-69447 Lyon cedex 03, Pharmacy Department: Drugs Fabrication and Control, E. Herriot Hospital, wing X, Place d’Arsonval, F-69437 Lyon cedex 03

Often presented as metabolism byproducts, reactive oxygen species are linked to detrimental effects such as chronic wound, mutagenesis, cancer and skin ageing. However, recent in vitro and in vivo observations suggest that ROS, and mainly hydrogen peroxide, interfere with cell signaling acting like second messenger and inducing adaptive responses. This is particularly observed in skin wound healing where cells are exposed to H 2O 2 following injury. In this study, we developed and characterized an innovative formulation producing H 2O 2 at low concentrations, in order to mimic physiological inflammation phase. Then, this pro-oxidative formulation (CAM-GOx) was assayed in vitro on keratinocytes cell culture, compared to the blank formulation (CAM) and the anti-oxidative formulation (CAM-CAT) to assess whether oxidative stress was implied or not in cellular responses.