John Libbey Eurotext

European Cytokine Network


LL-37, HNP-1, and HBD2/3 modulate the secretion of cytokines TNF-α, IL-6, IFN-γ, IL-10 and MMP1 in human primary cell cultures Volume 27, numéro 3, September 2016


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1 Medical Research Unit of Zacatecas, Mexican Institute of Social Security, Zacatecas, Mexico
2 Faculty of Chemical Sciences, Autonomous University of Zacatecas, Zacatecas, Mexico
3 Research Center for Health Sciances and Medicine, San Luis Potosí, México
* Correspondence: Mariana H. García-Hernández, Medical Research-Unit of Zacatecas. Mexican Institute of Social Security-IMSS. Interior Alameda No. 45. 98000 Zacatecas, Zac, México
  • Mots-clés : host defense peptides, inflammatory cytokines, matrix metalloproteinases, rheumatoid arthritis, chondrocyte culture, neutrophil culture
  • DOI : 10.1684/ecn.2016.0379
  • Page(s) : 68-74
  • Année de parution : 2016

The aim of this study was to evaluate the effects of the LL-37, HNP-1 and HBD2/3 peptides on cytokine and MMP production in human polymorphonuclear cells, mononuclear cells and chondrocytes. The levels of cytokines in supernatants from mononuclear and polymorphonuclear cell cultures were measured with a cytometric bead array by flow cytometry. Likewise, the levels of metalloproteinase/MMP-1, 3, and 13 were measured in supernatants from chondrocyte cultures using an ELISA. The expression of RANKL on lymphocytes was analyzed by flow cytometry. We observed increased levels of TNF-α, IL-6 and IL-10 in mononuclear and polymorphonuclear cell cultures stimulated with HBD-2/3. We also observed increased levels of IFN-γ, IL-10, and IL-6 in mononuclear cell cultures stimulated with HNP-1, and increased IL-6 levels were observed in polymorphonuclear cell cultures exposed to HNP-1. We also found that the MMP-1 level increased in the chondrocyte cultures stimulated with HBD-3, whereas the MMP-1 level was decreased in cultures exposed to LL-37. The present report is the first study to determine that HNP-1and HBD2/3 promote the secretion of pro-inflammatory cytokines by polymorphonuclear and mononuclear cells and the secretion of MMP by chondrocytes, whereas LL-37 diminishes MMP1 secretion. Our results suggest that HBD-2/3 and HNP1 might play a pathological role in rheumatoid arthritis, while LL-37 might have a protective role.