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European Journal of Dermatology

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Role of the long non-coding RNA, SPRR2C, based on an in vitro psoriatic keratinocyte cell model Volume 32, numéro 2, March-April 2022

Illustrations


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Tableaux

Auteurs
1 Department of Dermatology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, PR China
2 Laboratory of Basic Medical Science, Qilu Hospital of Shandong University, Jinan 250012, Shandong, PR China
3 Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, PR China
Reprints: Qing Sun
a These authors contributed equally

Background

Psoriasis is a chronic inflammatory disease of the skin with complex pathogenesis. Long non-coding RNAs (lncRNAs) play an important regulatory role in the occurrence and progression of many diseases, as well as psoriasis.

Objectives

This study aimed to investigate the role and mechanism of the lncRNA, SPRR2C, in M5-induced psoriatic keratinocytes.

Materials & Methods

SPRR2C expression and subcellular localization was detected using FISH and qRT-PCR. Ker-CT and HaCaT cells stimulated by M5 (IL-17A, tumour necrosis factor-α, IL-1α, IL-22, and oncostatin-M) were used to establish a psoriatic cell model. CCK-8 assay, CFSE proliferation assay, flow cytometry, western blotting and ELISA were used to examine the effects of SPRR2C in the keratinocyte model.

Results

SPRR2C was highly expressed in psoriatic samples and M5-induced psoriatic keratinocytes, and SPRR2C was mainly localised to the cytoplasm. In keratinocytes, SPRR2C regulated proliferation, cell cycle and apoptosis, and induced the expression of IL-1β, IL-6, IL-8, CXCL2 and CCL20. Moreover, SPRR2C cellular effects were shown to be mediated by the PI3K/AKT/mTOR signalling pathway, based on experiments with the AKT-specific inhibitor, MK-2206, which was also shown to suppress overexpression of SPRR2C.

Conclusion

Our results indicate that SPRR2C plays a regulatory role and is involved in the PI3K/AKT/mTOR signalling pathway in psoriatic keratinocytes, which may provide a potential diagnostic and therapeutic target for psoriasis.