CIRI (International Center for Infectiology Research) INSERM U1111, Ecole Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, CNRS UMR5308, 21 avenue Tony Garnier, 69007 Lyon, France
DERMATEC SAS, Espace Lamartine, 9 avenue Victor Hugo, 69160 Tassin la demi-lune, France
Lefebvre Tiphaine, 6 route de Sécheron, 73000 Jacob-Bellecombette, France
SIGVARIS SAS, Z.I. Sud d’Andrézieux Rue Barthélémy Thimonnier, 42170 St-Just St-Rambert Cedex, France
Allergy and Clinical Immunology Department, Lyon Sud University Hospital, 165 chemin Grand Revoyet, 69495 Pierre Bénite cedex, France
These authors contributed equally.
Background: Chronic venous disease (CVD) is secondary to venous hypertension, leading to vascular inflammation and tissue changes. The impact of CVD on skin structure and barrier function is not well characterized. Objective: We aimed to assess the characteristics of skin alterations in mild-to-moderate CVD by non-invasive techniques based on a prospective exploratory study. Material & Methods: Female subjects (30-75 years) with CVD (Stage C2 to C4, CEAP classification) were eligible. Stage C0-C1 CVD subjects were used as controls. Women with leg surgery or a medical history that could impact the results were excluded. The skin changes on lesional (LS) and non-lesional (NLS) areas were assessed by biometric analysis including skin echography, viscoelasticity evaluation, confocal microscopy and trans epidermal water loss (TEWL) measurements. Results: Thirty-four subjects were enrolled. Based on computation of 26 biometric parameters using Principal Component Analysis, a significant difference between LS and NLS zones, regardless of the CEAP class, was evidenced. C2-C4 subjects presented with dermal thickening suggesting oedema associated with decreased cell density, while no difference in skin viscoelasticity was observed compared to the C0-C1 control group. Epidermal structural modifications were associated with increased TEWL correlating with CVD severity. Conclusion:Skin alterations in CVD patients are detectable by non-invasive methods. These findings may help to better assess new therapeutic strategies.