Figure 2
Neuropathological and immunohistochemical analysis. (A) Fragment of brain tissue containing the calcified granuloma. An amplified image shows cresyl-violet staining of the lesion. Insets, a and b, represent proximal (up to 200 μm from the NCC fibrous wall) and distal (more than 200 μm from the NCC fibrous wall) cortex regions, respectively, for which the IHC analysis was performed.(B) Evaluation of immunohistochemical markers in representative slices from different regions (left panel: x5 magnification; other panels: x10 magnification). (C) Immunofluorescence (magnification: x40); note the marked increase in microglial activation in the necrotic and proximal regions.