JLE

Annales de Biologie Clinique

MENU

Evaluation of HBA1C measurement on Architect CI8200 (Abbott Diagnostic). Comparison with HPLC D-10 Bio-Rad assay Volume 67, issue 1, janvier-février 2009

Figures

See all figures

Authors
Laboratoire de biochimie,, Service information et évaluation médicale,, Unité de semaine diabétologie- endocrinologie, Centre hospitalier de la Région d’Annecy, Pringy

The aim of this study is to present an evaluation of HbA 1c Assay on Architect CI8200 (Abbott Diagnostic). The measurement includes Hb assay by colorimetry and HbA 1c by immunoturbidimetry. The percentage of HbA 1c is the report HbA 1c/complete Hb with a conversion coefficient. Repetability (n = 30; CV: 1,15-1,91%) and reproductibility (n = 30; CV: 2,09-2,64%) are good. Abbott results cannot be returned above 12%. Comparison between HbA 1c Abbott and HbA 1c Bio-Rad is performed on 161 patients samples ranging from 4,7 and 12%. Results show a correlation coefficient of 0,9847 (N = 161) with a regression equation: [Abbott] = 1,02x [Bio-Rad]-0,636]. Differences between two methods are normally distributed. 95% of differences lie between limits (-0,61%; +0,61%). Such differences are clinically important and interchangability of two measurements can’t be possible now because lack of agreement. We hope that IFCC standardization will reduce these differences. Presence of a jaundice and carbamylation of haemoglobin do not interfer with Abbott assay. Hemoglobin variants are not detected. Therefore, monitoring of diabetic patients with HbA 1c is possible only if hemoglobinopathy has been identified before.