John Libbey Eurotext

Cahiers d'études et de recherches francophones / Santé

Description d’une méthode simple de mesure de la parasitémie palustre Volume 1, numéro 4, Octobre-Novembre 1991

Auteurs
Département de parasitologie, chargé de recherche à l’ORSTOM, Centre Pasteur du Cameroun, B.P. 1274, Yaoundé, Cameroun, Département de parasitologie, Faculté des Sciences de la Santé, B.P. 188, Cotonou, Bénin.
  • Page(s) : 324
  • Année de parution : 1991

A simple, rapid and cheap method for evaluation of malarial parasitaemia is described for epidemiological and morbidity surveys in endemic areas. Malarial parasite density is obtained by thin smear and based upon individual number of red cells determination using a microhematocrit measurement (10,000 rounds par minute during 5 minutes). Parasitaemia per micro-liter of blood is given by the formula: P = H (0,085 X + 0,913) x 106 / 280 N. H is the number of infected red cells, X is the value of micro-hematocrit and N is the number of examined fields. Mean number of red cells per field was defined after red cells numeration in 500 microscopic fields at 1000x magnification. Number of examined fields can be, with sufficient precision, 75 or 100 for each thin smear. The parasite density threshold obtained is about 150 parasites per microliter.