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 Version imprimable |
TLR expression in human melanoma cells |
European Journal of Dermatology. Volume 21, Numéro 6, 899-905, November-December 2011, Investigative report
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Auteur(s) : Mélanie Saint-Jean, Anne-Chantal Knol, Jean-Michel Nguyen, Amir Khammari, Brigitte Dréno |
Résumé : There is increasing evidence that melanoma cells express TLR2, -3, and -4. However, the expression of other TLRs by these cells still remains unknown. We investigated the expression patterns of TLR2, -3, -4, -7, -8 and -9 both on melanoma-invaded lymph nodes and on melanoma cell lines. TLR2, -3, -4, -7 and -9 mRNA expression was determined by quantitative RT-PCR. The TLR protein expression level was measured
ex vivo by immunohistochemistry and
in vitro by flow cytometry.
Results: At the mRNA level, melanoma cells
in vitro, and possibly
ex vivo, expressed TLR2, -3, -4, -7 and -9. TLR2 and -4 protein expressions
ex vivo were over 50%, contrasting with an absence of these 2 TLRs
in vitro. On the contrary, TLR-3 and -8 proteins had a low expression
ex vivo with a
high expression
in vitro. TLR-7 and -9 proteins were expressed
ex vivo and
in vitro. Our study demonstrates for the first time that melanoma cells express TLR7 and -8. |
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