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 Version imprimable |
Staphylococcus aureus isolated from patients with recurrent furunculosis carrying Panton-Valentine leukocidin genes represent
agr specificity group IV |
European Journal of Dermatology. Volume 21, Numéro 1, 43-6, January-February 2011, Investigative report
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 Article gratuit
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Auteur(s) : Katarzyna GARBACZ, Lidia PIECHOWICZ, Wioletta BARAŃSKA-RYBAK, Maria DąBROWSKA-SZPONAR |
Résumé : Recurrent furunculosis (RF) caused by
Staphylococcus aureus presents a difficult clinical problem and causes significant morbidity. The study aim was to characterise
agr groups and detect toxin genes among
S. aureus strains isolated from RF patients. Microbiological material was obtained from evacuated furuncules of 44 RF patients. Nasal swabs were obtained from both the RF patients and the controls (150 healthy volunteers with no history of RF). All strains were screened for the presence of
lukS/lukF-PV,
tst,
sea,
seb,
sec,
sed,
eta, and
etb genes. Moreover,
agr specificity groups (I-IV) were identified. Antibiotic-susceptibility tests were performed by disk diffusion method and methicillin susceptibility was verified by
mecA gene amplification. The investigated strains were resistant to penicillin, clindamycin, erythromycin, and tetracycline. All showed susceptibility to methicillin. Thirty-five of 44 strains tested were positive for leukocidin
lukS/lukF-PV genes and 12/44 for enterotoxin
seb gene. The coexistence of PVL genes and
seb gene concerned 7/44 strains. The remaining toxin genes were not found. Forty-three strains belonged to
agr specificity group IV including all strains with
lukS/lukF-PV genes. Nasal carriage of
S. aureus was observed in 27/44 (61.3%) RF patients and in 43/150 (28.6%) controls (
p \= 0.001). In all RF subjects, nasal strains did not differ from those isolated from furuncules in terms of
lukS/lukF-PV gene status and
agr specificity. To the best of our knowledge, it is the first study that shown such a predominance of
agr group IV strains in RF patients. |
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