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European Journal of Dermatology

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The NC1 domain of type XIX collagen inhibits melanoma cell migration Volume 20, numéro 6, November-December 2010

Auteurs
CNRS UMR 6237, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51095 Reims, France, Laboratoire Central de Biochimie, CHU de Reims, rue Serge Kochman, 51092 Reims, France, IFR 53, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51095 Reims, France, CNRS UMR 6229, Université de Reims Champagne-Ardenne, 51 rue Cognacq Jay, 51095 Reims, France

Type XIX collagen is a minor collagen that localizes to basement membrane zones. We previously demonstrated that the C-terminal NC1 domain of type XIX collagen inhibits tumor growth in vivo. In the present study, we analyzed the effects of the NC1(XIX) collagen domain on migratory behaviour of melanoma B16F10 cells. We found that NC1(XIX) do not inhibit melanoma cell proliferation. On the contrary, NC1(XIX) strongly inhibited the migratory capacities of melanoma cells in the scratch wound model and in Ibidi ® devices: cell migration speed was 7.69 ± 1.49 μm/h for the controls vs 6.64 ± 0.82 μm/h for cells incubated with 30 μmol/L NC1(XIX) and 5.72 ± 0.67 μmol/h with 60 μmol/L NC1(XIX). Similar results were obtained with UACC 903 human melanoma cells. Further work will be necessary to elucidate the molecular mechanisms of this migration inhibition. It may, however, explain, at least partially, the inhibition of tumor growth that we observed in vivo.