European Cytokine Network
MENUTNF-α messenger ribonucleic acid (mRNA) in patients with nonalcoholic steatohepatitis Volume 23, numéro 3, July-August-September 2012
Illustrations
- Mots-clés : nonalcoholic steatohepatitis, tumor necrosis factor, RNA, PCR
- DOI : 10.1684/ecn.2012.0313
- Page(s) : 107-11
- Année de parution : 2012
<p>tumor necrosis factor (TNF)-α plays a significant role in the pathogenesis of nonalcoholic steatohepatitis (NASH). A few studies have confirmed high TNF-α plasma protein levels in patients with NASH compared to healthy volunteers. We herein aimed to revisit these findings using other molecular techniques.</p><p>a cross-sectional evaluation of patients newly diagnosed with NASH. A quantitative assay for the measurement of TNF-α messenger ribonucleic acid (mRNA) was performed for NASH patients and controls using real-time reverse transcription polymerase chain reaction (RT-PCR).</p><p>in 39 patients with NASH (mean age 38.6 ± 9.4 years, range 28-60 years; 79% males), the mean TNF-α mRNA level was significantly higher than that found for controls (137.6 ± 102.3 ng/mL versus 83.5 ± 43.8 ng/mL, respectively; P = 0.012). A TNF-α mRNA cut-off of 100 ng/mL predicted NASH most optimally (AUC 0.685 ± 0.066, P = 0.01; with 66.7% sensitivity and 74.1% specificity). Serum TNF-α and soluble TNF-α receptor II (sTNFRII) levels were significantly higher in patients compared to controls using ELISA.</p><p>high TNF-α mRNA levels, determined by RT-PCR, characterize patients with NASH.</p>