John Libbey Eurotext

European Cytokine Network

Increased serum IL-17A and Th2 cytokine levels in patients with severe uncontrolled asthma Volume 28, numéro 1, March 2017

Figure 1

Serum IL-17A levels determined by ELISA. A. Serum IL-17A levels (pg/mL) were compared in relation with the control status, history of systemic steroid therapy, and emergency room (ER) admission within 12 months. B. Serum IL-17A levels were compared between patient groups with controlled and uncontrolled asthma, in relation with daily medication steps and ICS doses. C. The correlation between %FEV1 and serum IL-17A levels. Results are shown as individual data points with median (bars) and interquartile range (box). Open circles indicate patients with controlled asthma and closed circles indicate patients with uncontrolled asthma. Open diamond indicates the patients without steroid history, and closed diamond indicates the patients with steroid history. Open triangle indicates the patients without ER history, and closed triangle indicates the patients with ER history. P-values were calculated using Welch's t-test (A, B).

Figure 3

Serum levels of IL-17A and other cytokines. A. The levels of IL-17A and related cytokines were compared between patient groups of atopic asthma (open circle) and no-atopic asthma (closed circle). B. The levels of serum cytokines were compared between patient groups with controlled (open circle) and uncontrolled asthma (closed circle) and between patient groups with atopic and non-atopic asthma. P-values were calculated using Welch's t-test.

Figure 4

Clustering patients using IL-17A and other cytokines. A. Unsupervised hierarchical clustering analysis of IL-17A and related cytokine levels. Cluster analysis was performed by complete linkage based on Euclidean distance. B. The patient characteristics, including daily medication step, control status, and occurrence of severe exacerbation within 12 months, were compared among the groups identified in A. Statistical significance was calculated using Fisher's exact test. C. The serum levels of cytokines among the four groups identified in A. Results are shown as individual data points with median (bar) and interquartile range (box). The groups are as follows: G1 (blue dot), G2 (green dot), G3 (orange dot), and G4 (red dot).

Figure 2

Correlation between IL-17A and other cytokines. The correlation diagram among IL-17A and IFN-γ, IL-4, IL-9, IL-10, and IL-25 was shown in all asthmatic patient group (A) and patient groups with controlled (B) and uncontrolled asthma (C). The correlation was calculated using Spearman's rank correlation.