John Libbey Eurotext

European Cytokine Network

Characterization of a long isoform of IL-1R8 (TIR8/SIGIRR) Volume 28, numéro 2, June 2017

Illustrations

  • Figure 1
  • Figure 2
  • Figure 3
  • Figure 4
Auteurs
Cell signaling unit, division of experimental oncology, IRCCS San Raffaele hospital, Milano, Italy
* Correspondence: Marta Muzio, cell signaling unit, division of experimental oncology, IRCCS San Raffaele hospital, via Olgettina 58, Milano, Italy.
  • Mots-clés : interleukin-1 receptor, Toll-like receptor, IL-1R8, TIR8, SIGIRR, splicing
  • DOI : 10.1684/ecn.2017.0395
  • Page(s) : 63-9
  • Année de parution : 2017

IL-1R8, also known as SIGIRR or TIR8, is a trans-membrane protein belonging to the IL-1 receptor family. The human gene includes ten exons, and alternative splicing can result in different isoforms. We, herein, characterized a longer isoform of IL-1R8 containing an in-frame additional sequence between the TIR domain and the C-terminal portion of the protein. IL-1R8 Long (IL-1R8L1) mRNA was specifically expressed and regulated in distinct cell lines, in a manner similar to the classic isoform. Overexpression of IL-1R8L1 resulted in the production of a corresponding protein that showed a pattern of cell localization similar to the classic isoform. An antibody directed against an IL-1R8L1 specific peptide, detected this novel isoform in different cell lines and tissues where this protein may complement the anti-inflammatory functions of classic IL-1R8.