Auteur(s) : I. Tong Mak, Jay H. Kramer, William B. Weglicki. , The George Washington University Medical Center, Department of Physiology and Experimental Medicine, Washington, DC 20037, USA .
Illustrations
Fig. 1. Basal (A) and PMA-stimulated (B)
superoxide anion generating activity in neutrophils isolated from
MgS or MgD rats with or without L-703,606 treatment. The rats
were fed MgS or MgD diets for 3 weeks with or without
receiving L-703,606 as s.c. implantation for the entire
period. The neutrophils were isolated as described in Materials and
Methods; the cells were incubated at 37° with or without challenge
by PMA (0.25 ug/ml). Superoxide anion producing activity was
determined as SOD-inhibitable cytochrome c reduction. Results are
means ± SD of 4-6 animal samples.
* P < 0.05 vs MgD, **
P < 0.01 vs MgS control.
Fig. 2. Effect of L-703,606 (in
vivo) on (A) plasma levels of NO products
(nitrite + nitrate) in MgS or MgD rats and (B) on
effluent nitric oxide product levels from postischemic hearts of
MgD and MgS rats. Plasma samples were quickly frozen
at – 80° for later NO product determination. Plasma data
are means ± SD of 4-5 animals; #
p < 0.001 vs MgS control; **
p < 0.01 vs MgD control. The hearts were
subjected to 30 min global ischemia/reperfusion. Effluent
samples were assessed for nitric oxide products (nitrite) using the
Griess reagent assay during the 15 min reperfusion period.
After normalizing to preischemic levels, values are reported as
total content during reperfusion. Values are means ± SE
of 8-10 hearts. # p < 0.001 vs all
other groups.
Fig. 3. Effect of L-703,606 on the plasma
levels of the PGI2 breakdown product, 6-keto-PG
F1α in the rat with or without Mg-deficiency for
3 weeks. The rats were fed MgS or MgD diets for 3 weeks
with or without receiving L-703,606 as s.c. implantation for
the entire period. Freshly isolated plasma samples (unfrozen) were
assayed for the levels of 6-keto-PG F1α as described in
the Methods. Results are means ± SD of 4-6 animals;
* p < 0.05 vs
MgS, + p < 0.02 vs MgD alone.
Version imprimable
