Physiology and Physiopathology Laboratory, University Pierre et Marie Curie, 4, Place Jussieu, 75252 Paris cedex 05, France; Centre d‘Etudes Nucléaires de Bordeaux‐Gradignan, Interface Physique‐Biologie, Le Haut Vrigneau, BP 130, 33175 Gradignan cedex, France; SDRM, 64 rue de Longchamp, 92200 Neuilly\Seine, France
- Key words: calcium, chloride, connective tissue, epithelial layer, human amniotic membrane, magnesium, nuclear microanalysis, potassium, sodium.
- Page(s) : 35-42
- Published in: 2003
The elemental ionic distribution in the epithelial layer (EL) and in connective tissue (CT ∓ compact layer + fibroblast layer) of the human amniotic membrane has been studied in reference samples, after conservation in a physiological fluid (Hanks‘solution) and after addition of 2 mM MgCl
2 in Hanks‘solution. Particle induced X‐ray emission and Rutherford backscattering spectrometry techniques were used to provide quantitative measurements. In physiological fluid, with regard to reference samples, the monovalent ions (Na
+, K +, Cl
‐‐ ) concentrations were identical on both layers. This data indicates that the connective tissue, in particular the compact layer, acts as a buffer which fix minerals. Mg
2+ and Ca
2+ levels were higher in EL than in CT. The addition of MgCl
2 in Hanks‘solution induced a decrease of the monovalent ion concentrations in both layers except Na
+ level in EL which remained constant, an increase of the Mg
2+ level in both layers, while the Ca
2+ remained constant. These data indicate the possible role of connective tissue in pregnancies complicated by poly or oligohydramnios.