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 Printable version |
Clinical applications of the detection of activated platelets using flow cytometry |
Sang Thrombose Vaisseaux. Volume 9, Number 7, 441-8, Septembre 1997, Mini-revues
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 Résumé
Article gratuit
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Author(s) : Paquita Nurden, Catherine Durrieu-Jaïs, Alan Nurden |
Summary : On of t he recent t echnol ogical advances in the study of arterial thrombosis is the detection of circulating activated platelets by flow cytometry. This procedure makes use of the property of platelets to express at their surface glycoproteins (GP), such as P-selectin or granulophysin, normally exclusive to the membrane of granules and inaccessible to antibodies in resting platelets. Activated platelets can also be identified using monoclonal antibodies specific for epitopes created or newly expressed in the GP IIb-IIIa complex during platelet activation. Bound antibodies are assessed on individual platelets by fluorescence measurements. Activated platelets have been detected in the circulation of patients at high risk for arterial thrombosis ; nevertheless their measurement for predicting thrombotic events remains controversial. Detection of activated platelets has been largely evaluated during or after percutaneous angioplasty or stent implantation in coronary arteries. In this context, it is a potentially valuable technique to evaluate the efficacity of new therapeutic agents blocking platelet surface glycoproteins. Flow cytometry has also been used to define the best conditions of conservation of platelet concentrates. It has much potential and will be used to explore other areas of platelet pathology in years to come. |
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