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 Printable version |
Genetic limits: which place for preimplantation genetic diagnosis? |
Médecine Thérapeutique / médecine de la reproduction. Volume 9, Number 6, 414-8, Novembre-Décembre 2007, Revue
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 Résumé
Article gratuit
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Author(s) : Samir Hamamah, Tal Anahory, Frank Pellestor, Vanessa Loup, Lionel Reyftman, Hervé Dechaud, Bernard Hedon |
Summary : The preimplantation genetic diagnosis (PGD) necessitates an in vitro fertilization (IVF) aiming at obtaining several embryos for genetic or chromosomal analysis. In France, it is restricted to fertile or subfertile couples at risk of transmitting a severe and non treatable disease. The embryo number must be sufficient enough to yield at least one unaffected embryo. This requires careful attention to the ovarian function and to the ovarian stimulation modalities. The intracytoplasmic sperm injection (ICSI) technique should nowadays be preferred for PGD. Embryo biopsy is usually performed at day 3 on embryos (6-8) cells, with the aim to aspirate one or two cells for a diagnosis by PCR or FISH, preferentially using 2 blastomeres from a single embryo. According to each center, setting up this technique may take from 6 to 9 months. The transfer policy should remain the same as in standard IVF to avoid multiple pregancy. The success of the attempt lies on the oocyte number, the number of embryos to biopsy and thus, the resulting number of unaffected embryos. |
Keywords : preimplantation genetic diagnosis, genetic disease, IVF, embryo, ICSI |
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