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 Printable version |
Intraembryonic hematopoietic stem cells in the mouse : even earlier |
Hématologie. Volume 3, Number 5, 417-22, Septembre-Octobre 1997, Mini-revues et revues
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 Résumé
Article gratuit
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Author(s) : Françoise Dieterlen-Lièvre, Ana Cumano, Isabelle Godin |
Summary : In a previous review [1], we reported how we had detected the appearance of intraembryonic hematopoietic progenitors in the mouse, using a two-step cell culture method. Progenitors with similar potentialities were shown to appear in parallel in the yolk sac and it was impossible to assign their primary origin to one or other of these regions. We have now moved back in development by introducing an organ culture phase prior to the two-step cell culture. Progenitor emergence could thus be demonstrated at the 0-10 pairs of somite stages (7,5-8,5 days post-coïtus), prior to blood circulation. The progenitors appear in parallel in the caudal intraembryonic splanchnopleural mesoderm and in the yolk sac mesoderm. However the hematopoietic potential of these two regions is different : only the embryo proper provides progenitors endowed with lymphoid potential. Furthermore progenitors of intraembryonic origin are capable of renewing in culture for a longer period than those from the yolk sac. |
Keywords : hemopoietic stem cells, mouse, intraembryonic splanchnopleura, yolk sac. |
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