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A new medium for diagnosis of dermatophyte infection

European Journal of Dermatology. Volume 19, Number 1, 34-7, January-February 2009, Investigative report

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Author(s) : Xiao-Fang Li, Yong-Nian Shen, Wei Chen, Hui Chen, Gui-Xia LV, Wei-Da Liu

Summary : A new medium (DBM) was compared with dermatophyte test medium (DTM) for the diagnosis of dermatophyte infection. The sensitivity was 10 3 cfu/mL (2 × 10 1 cfu/slant) for both DTM and DBM with a suspension of Trichophyton rubrum. In axenic cultures, all dermatophytes tested altered the color of both media. Although most non dermatophytic molds made a color change, it was at a slower rate. In nail samples of dermatophyte infection, all dermatophytes altered the color of both media. However, the time for discoloration was shorter with DBM than with DTM (5.83 ± 0.39 days vs. 7.32 ± 0.41 days, t = 2.63, P = 0.01). Most isolates of nondermatophyte also made a discoloration, but they could be distinguished from dermatophytes by their colonial diameters when the color began to change (≥ 5 mm). Our results were in good agreement with a professional laboratory of medical mycology, however, the latter is regularly able to differentiate exactly the species of the growing dermatophyte. The DBM medium is more convenient, rapid, more accurate and economical to use than DTM.

Keywords : culture, dermatophytes, dermatophyte test medium (DTM), diagnosis, dermatophyte infection


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