|
|
 |
 |
| |
 Printable version |
Effects of date seed oil on normal human skin in vitro |
European Journal of Dermatology. Volume 17, Number 6, 516-9, November-December 2007, Investigative report
|
 Free Article
|
Author(s) : Ines Dammak, Fatma Ben Abdallah, Sonia Boudaya, Laila Keskes, Souhail Besbes, Amel El Gaied, Hamadi Attia, Hamida Turki, Basma Hentati |
Summary : Oxidative stress has been implicated in various skin diseases through the generation of reactive oxygen species and the depletion of endogenous antioxidant systems. The administration of antioxidants or free radical scavengers is reportedly helpful, notably in order to enhance the healing process. We investigated the protective effect of a new natural product: date seed oil: (DSO) against hydrogen peroxide (H
2O
2)-induced oxidative stress, in terms of lipid peroxidation, depletion of endogenous antioxidant defense enzymes such as glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), one day after a 2 h exposure to H
2O
2, using human skin organ culture as an in vitro model. In the investigated model system, DSO has significant protective effect, by inhibition of damage caused by H
2O
2, endowing a radical scavenging ability. Treatment of skin with DSO inhibited H
2O
2-induced lipid peroxidation. In addition, this oil inhibited H
2O
2-induced depletion of antioxidant defense components, such as superoxide dismutase and catalase. We conclude that DSO could be useful in the attenuation of H
2O
2-induced oxidative stress-mediated skin diseases in human skin, possibly due to antioxidant properties. |
Keywords : catalase, date seed oil, glutathione peroxidase, MDA, superoxide dismutase |
|
