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 Printable version |
In vitro expression of members of the interleukin-1 family by dermal papilla cells and possible implications for alopecia areata |
European Journal of Dermatology. Volume 7, Number 2, 103-7, March 1997, Revues
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 Free Article
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Author(s) : A. Steins, D. Zuder, M. Hahn, T. Klyscz, M. Jünger, G. Rassner |
Summary : Recent data suggest that hair loss in alopecia areata (AA) may be triggered by cytokines such as IL-1. The dermal papilla is a likely target in AA, but little is known about the immune properties of dermal papilla cells (DPC). For this reason, FACS and ELISA analysis and semiquantitative RT-PCR were used to determine constitutive and cytokine-regulated expression of IL-1 receptors as well as IL-1b and IL-1RA in dermal papilla cells. IL-1b and IL-1-RA were detected in cell lysates only. Stimulation with phorbolester, IL-1b or TNF-a led to a time-dependent increase in both proteins. By use of RT-PCR, transcripts for IL-1b, s-IL-1RA and ic-IL-1RA were detected, but only IL-1b and ic-IL-1RA transcripts were upregulated after treatment with PMA, IL-1b or TNF-a. Other factors such as IFN-g, IL-2, IL-4, IL-6, IL-8, IL-10, GM-CSF, IL-13 or TGF-b1 had no effect on constitutive or elicited IL-1b or IL-1RA expression. Remarkably, PGE2 was a selective inducer of IL-1-RA protein. IL-1 receptors of both type I and type II were present on dermal papilla cells in vitro and their surface expression was markedly enhanced by co-stimulation with IFN-g and TNF-a, but remained unaffected by any other treatment. Our results show that the dermal papilla constitutes an immune microenvironment that is equipped to participate in IL-1-driven immune responses. The presence of IL-1-RA and IL-1 receptors within the hair bulb may reflect a pivotal mechanism by which DPC are able to tune or even neutralize harmful lesional IL-1 levels in cases of follicular or perifollicular inflammation. |
Keywords : alopecia areata, cytokines, hair, IL-1. |
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