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 Printable version |
In vitro released interferon-gamma in the diagnosis of drug-induced anaphylaxis |
European Journal of Dermatology. Volume 9, Number 7, 559-60, October - November 1999, Cas cliniques
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 Free Article
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Author(s) : Y. Kubota, T. Koga, J. Nakayama |
Summary : A 17-year-old Japanese male was referred with acute urticaria and anaphylaxis after the administration of PL® (salicylamide, acetaminophen, anhydrous caffeine and promethazine methylene disalicylate) and Bufferin® (aspirin and dialminate) for headache and a high grade fever. The results of prick test, patch test and drug-induced lymphocyte stimulation test with PL® and Bufferin® were all negative. The patient’s peripheral blood mononuclear cells (PBMC) were cultured with or without PL® for 72 hours, and the activity of interferon-gamma (IFN-gamma) in the culture supernatant was measured with EIA. A significantly high level of IFN-gamma was detected in PBMC from the patient, but very little in those from healthy control subjects with a history of exposure to PL®. This finding may indicate the presence of drug-specific IFN-gamma producing T cells in patients with an anaphylactic shock reaction to medication. Assays that measure the drug-induced IFN-gamma production may thus be a useful diagnostic tool not only for identifying delayed-type hypersensitivity (DTH) to drugs, but also for predicting anaphylactic shock reaction to drugs. |
Keywords : drug reactions, interferon-gamma production test, in vitro diagnosis ; drug-induced anaphylaxis, PL®. |
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