|
|
 |
 |
| |
 Printable version |
Detection and sequence diversity of Kaposi’s sarcoma-associated herpesvirus (KSHV)/human herpesvirus 8 (HHV-8) DNA |
European Journal of Dermatology. Volume 9, Number 1, 13-6, January - February 1999, Revues
|
 Free Article
|
Author(s) : Mari MURAKAMI, Yasuyuki SUGITA, Norihisa ISHII, Tetsuo NAGATANI, Shinichi ICHIYAMA, Nobuko TAMURA, Shinobu MOHRI, Hiroshi NAKAJIMA |
Summary : Detection of Kaposi’s sarcoma (KS)-associated herpesvirus (KSHV)/human herpesvirus (HHV-8) has been reported frequently in patients with KS associated with acquired immunodeficiency syndrome (AIDS). We examined the presence of the KSHV sequence in 8 HIV-positive patients comprising 5 with KS, 2 with syphilis, 1 with prurigo, and 2 HIV-negative patients with angiosarcoma. Using the polymerase chain reaction, we observed amplification of a DNA fragment of the expected size in 4 patients with KS. Sequencing analysis of the amplified fragments revealed several base substitutions upon comparison with the originally reported sequence. Our results support the hypothesis of a pathogenic role of KSHV in the development of skin lesions in HIV-positive patients with KS, and the sequences of KSHV DNA fragments isolated in this study also demonstrated strain diversity similar to that reported previously. |
Keywords : KSHV/HHV-8, Kaposi’s sarcoma, DNA sequence. |
|
