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 Printable version |
Lipoteichoic acid of
Bifidobacterium in combination with 5-fluorouracil inhibit tumor growth and relieve the immunosuppression |
Bulletin du Cancer. Volume 99, Number 5, 55-63, Mai 2012, Electronic journal of oncology
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 Full Text
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Author(s) : Ning Xie, Yue Wang, Qiang Wang, Fu-rong Li, Bin Guo |
Summary : To investigate the therapeutic efficacy of lipoteichoic acid of
Bifidobacterium (BLTA) in combination with 5-fluorouracil (5-FU) treatment on the mice bearing inoculated hepatoma-22 (H
22) cells and the effects of BLTA on immunological regulation of organism, and explore its mechanisms.
Methods. Tumor-bearing mice were treated with 5-FU alone, BLTA alone or BLTA in combination with 5-FU. The tumor size were observed and measured regularly. The growth inhibiting rate (IR) of tumor was detected. MTT assay was used to evaluate the proliferation of T lymphocytes and splenic NK cell and CTL activity. Enzyme linked immunosorbent assay (ELISA) was used to detect the change of IFN-γ. FCM was used to detect T subgroup ratio of spleen cells of tumor-bearing mice. Expression change of mRNA and proteins of Foxp3 and TIM-3 were detected by Real-Time-PCR and Western blot in tumor-bearing mice tumor tissue.
Results. Both 5-FU and BLTA had inhibition effect on tumor-growth. While in the 5-FU + BLTA group, the inhibition of tumor growth was more significant, with increased T lymphocyte proliferation and IFN-γproduction of spleen cells. Spleen cells of tumor-bearing mice had high CD4
+CD25
+regulatory T cell (CD4
+CD25
+T
reg) ratio and high mRNA and proteins expression of Foxp3 and TIM-3, but in the BLTA and 5-FU group, CD4
+CD25
+T
reg ratio degraded, with down regulation mRNA and proteins expression of Foxp3 and TIM-3. But CD4
+ T cells also decreased in spleen cells of tumor-bearing mice by alone 5-FU treated, splenic NK cell and CTL activity also degraded, while CD4
+ T cells and splenic NK cell and CTL activity significantly increased by BLTA treated. BLTA in combination with 5-FU could also enhance the ratio of CD4
+ T cells and splenic NK cell and CTL activity.
Conclusion. The present study suggested that BLTA in combination with 5-FU could enhance antitumor effect, with inhibiting TIM-3/TIM-3L pathway, cutting down immunosuppressive activity of CD4
+CD25
+ T
reg and enhancing cell-mediated immunity. |
Keywords :
Bifidobacterium, lipoteichoic acid, 5-fluorouracil, immune function |
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