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 Printable version |
The expression and effects the CABYR-c transcript of
CABYR gene in hepatocellular carcinoma |
Bulletin du Cancer. Volume 99, Number 3, 26-33, Mars 2012, Electronic journal of oncology
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 Full Text
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Author(s) : Hua Li, Ling Fang, Xigang Xiao, Lei Shen |
Summary :
Background and aim. CABYR, a calcium-binding tyrosine phosphorylation regulated fibrous sheath protein, was initially reported to be testis-specific and subsequently shown to be present in brain tumors, pancreas cancer and lung cancer. This study aimed to investigate the expression and effects of the CABYR-c transcript of
CABYR gene in hepatocellular carcinoma.
Methods. mRNA and protein expression of CABYR-c was examined in 20 paired hepatocellular carcinoma tissues and adjacent non-cancerous tissues by real-time quantitative RT-polymerase chain reaction (PCR) and western blot analysis respectively. HepG2 cells were treated with the antisense oligodeoxynucleotides targeting CABYR-c mRNA (CABYR-c antisense oligonucleotides [AS ODNs]) for indicated times, the AS ODNs inhibition effect was evaluated by measuring the CABYR-c mRNA expression level of HepG2 cells after treatment using real-time quantitative RT-PCR, then cell proliferation was studied using MTT assay, and cell cycle distribution and apoptosis were detected by flow cytometry as well.
Results. CABYR-c mRNA levels in hepatocellular carcinoma tissues were significantly higher than that in the paired adjacent non-cancerous tissues (27.5 ± 1.2
versus 2.5 ± 0. 9,
P <\; 0.01). CABYR-c protein expression level in hepatocellular carcinoma tissues was also significantly higher than that in adjacent non-cancerous tissues. CABYR-c mRNA expression in HepG2 cells was most effective down-regulated after treatment of 600 nM CABYR-c AS ODNs for 48 h, which was selected for subsequent experiments. Incubation with 600 nM CABYR-c AS ODNs inhibited the cell growth of HepG2 cells in a dose- and time-dependent manner. The maximum inhibitory effect achieved at 600 nM after 72 h treatment (30.92 ± 3.25%,
P <\; 0.01). HepG2 cells treated 600 nM CABYR-c AS ODNs for 48 h exhibited an increasing proportion of cells in G0/G1 phase (
P <\; 0.05) and a decreasing proportion of cells in S phase (
P <\; 0.05), compared with untreated controls. No obvious differences were observed in G2/M phase. The fraction of apoptotic HepG2 cells in CABYR-c AS ODNs treated group was less than that of untreated control group (
P <\; 0.05).
Conclusion. CABYR-c is highly expressed in hepatocellular carcinoma tissues and may play an oncogenic role in heptocarcinogenesis as well as its progression. |
Keywords : CABYR-c, antiapoptosis, antisense oligonucleotides, HepG2, hepatocellular carcinoma |
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