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European Journal of Dermatology

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Increased expression of cellular FLICE/caspase-8 inhibitory protein in condyloma acuminatum Volume 21, issue 4, July-August 2011

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Authors
Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University, Wuhan Hubei 430022, PR China, Department of Dermatology, People's Hospital of Zhengzhou, Zhengzhou Henan 450003, PR China, Department of Immunology, Union Hospital, Tongji Medical College, Huazhong University, Wuhan Hubei 430022, PR China

Condyloma acuminatum (CA) is a benign epithelial tumor caused by infection with human papillomaviruses (HPVs) and characterized by abnormal cell proliferation. Cellular caspase-8 (FLICE)-like inhibitory protein (c-FLIP) was originally identified as an inhibitor of death-receptor signaling through competition with caspase-8 for recruitment to FAS-associated via death domain (FADD). More recently, it has been determined that c-FLIP is associated with the survival and proliferation of T cells and keratinocytes. The aim of this work was to study the expression of c-FLIP in CA and its relationship with keratinocyte proliferation. Immunoperoxidase staining methods were applied to analyze the location and expressions of both c-FLIP and proliferating cell nuclear antigens (PCNA) in 34 CA and 16 normal foreskin tissues. Semiquantitative reverse transcriptase-polymerase chain reactions (RT-PCR) and western blotting were performed to further identify the expression of c-FLIP in CA. c-FLIP expression at both mRNA and protein level was significantly higher in CA than normal foreskin. c-FLIP expression was highly correlated with the PCNA labeling index (LI) in CA. We concluded that c-FLIP overexpression might take part in keratinocyte proliferation in CA.