Laboratoires Pharmaceutiques de la Roche-Posay, La Roche-Posay, 11, avenue Dubonnet, 92400 Levallois-Perret, France.
- Key words: B7-2 (CD86), ICAM-1 (CD54), Langerhans cells, migration, spa water.
- Page(s) : 339-42
- Published in: 2000
An inhibitory effect of a spa water on keratinocyte-derived inflammatory cytokine production, as well as a suppressive activity of this thermal water on the allostimulatory capacity of epidermal Langerhans cells have been recently reported. The aim of this study was to investigate the in vitro effect of the spa water on the modulation of the Langerhans cell phenotype, as well as their migratory and stimulatory capacities. After treatment with 5 mM TNBS, we found that the number of migrating Langerhans cells was significantly lower in RPMI reconstituted with thermal water (RPMI-RP) than in RPMI reconstituted with distilled water, referred to as control medium (RPMI-C). A significant downregulation of both HLA-DR and costimulatory molecules, B7-2 and ICAM-1, has been observed on Langerhans cells after a 3 day culture in the spa water. However, when added to a mixed epidermal cell-lymphocyte culture, the thermal water did not significantly affect the allostimulatory activity of human Langerhans cells. Furthermore, a 2 day treatment in the thermal water did not alter the ability of the Langerhans cells to mount a hapten-dependent, T cell response. In conclusion, the thermal water from La Roche Posay did not affect the stimulatory function of human Langerhans cells whereas it significantly decreased their in vitro migratory properties. These results could explain, at least partly, the beneficial effect of the thermal water in the treatment of inflammatory skin diseases.