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 Printable version |
Physiological importance of the connective tissue in the human amnion. Role of magnesium |
Magnesium Research. Volume 16, Number 1, 35-42, March 2003, ORIGINAL ARTICLE
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 Free Article
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Author(s) : Michel Bara, Philippe Moretto, Jean Durlach, Andrée Guiet‐Bara |
Summary : The elemental ionic distribution in the epithelial layer (EL) and in connective tissue (CT ∓ compact layer + fibroblast layer) of the human amniotic membrane has been studied in reference samples, after conservation in a physiological fluid (Hanks‘solution) and after addition of 2 mM MgCl
2 in Hanks‘solution. Particle induced X‐ray emission and Rutherford backscattering spectrometry techniques were used to provide quantitative measurements. In physiological fluid, with regard to reference samples, the monovalent ions (Na
+, K +, Cl
‐‐ ) concentrations were identical on both layers. This data indicates that the connective tissue, in particular the compact layer, acts as a buffer which fix minerals. Mg
2+ and Ca
2+ levels were higher in EL than in CT. The addition of MgCl
2 in Hanks‘solution induced a decrease of the monovalent ion concentrations in both layers except Na
+ level in EL which remained constant, an increase of the Mg
2+ level in both layers, while the Ca
2+ remained constant. These data indicate the possible role of connective tissue in pregnancies complicated by poly or oligohydramnios. |
Keywords : calcium, chloride, connective tissue, epithelial layer, human amniotic membrane, magnesium, nuclear microanalysis, potassium, sodium. |
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