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Functional interleukin-10 promoter variants in coronary artery disease patients in Tunisia |
European Cytokine Network. Volume 21, Number 2, 136-41, June 2010, research article
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Author(s) : Sonia Ben-Hadj-Khalifa, Lakhdar Ghazouani, Nesrine Abboud, Ali Ben-Khalfallah, Fatma Annabi, Faouzi Addad, Wassim Y Almawi, Touhami Mahjoub |
Summary : ObjectivesThe contribution of interleukin (IL)-10 promoter variants -1082G/A, -819C/T, and -592C/A to the risk of coronary artery disease (CAD) was investigated in 291 CAD patients and 291 age- and gender-matched control subjects.Methods and resultsIL-10 genotyping was performed using PCR-allele-specific amplification (PCR-ASA). Regression analysis was employed in assessing the contribution of the IL-10 variants to the overall CAD risk. A higher frequency of the -592A allele (p \= 0.004), but not the -1082A (p \= 0.828) or -819T (p \= 0.952) alleles, was seen in CAD patients. A higher frequency of -592C/A (p \= 0.011), and a lower frequency of -592C/C (p \= 0.015) genotypes was noted in patients compared to healthy controls. Regression analysis demonstrated an association of -592C/A [OR (95% CI) \= 1.82 (1.02-3.23)] and -592A/A [OR (95% CI) \= 3.33 (1.27-9.09)] genotypes with 1-artery disease. Haplotype analysis revealed that none of the eight possible IL-10 haplotypes was associated with CAD or with the severity of CAD, and was confirmed by multivariate regression analysis, after adjusting for a number of confounders (smoking, systolic and diastolic blood pressure, hypertension, diabetes, glucose, cholesterol, and triglycerides).ConclusionsOur results suggest that the -592C/A, more so than the -1082G/A or the -819C/T IL-10 promoter variant alleles, may be considered to be a risk factor for CAD in Tunisians. |
Keywords : interleukin-10, PCR, gene polymorphism, coronary artery disease |
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