|
|
 |
 |
| |
 Printable version |
Synergistic inhibition of pseudorabies virus replication by porcine alpha/beta interferon and gamma interferon in vitro |
European Cytokine Network. Volume 18, Number 2, 23-9, June 2007, Research papers
|
 Free Article
|
Author(s) : Qingxia Yao, Ping Qian, Yi Cao, Yannan He, Youhui Si, Zhuofei Xu, Huanchun Chen |
Summary : Interferon (IFN) is crucial for initiating the innate immune response and for the generation of the adaptive response. IFN, in most species, comprises IFN-alpha (IFN-α), IFN-beta (IFN-β) and IFN-gamma (IFN-γ). In this study, we compared the capacity of porcine IFN-α, -β and -γ, or a combination of them, to protect IBRS-2 cells (porcine kidney cells) from infection with pseudorabies virus (PRV). The results demonstrated that porcine IFN-β (PoIFN-β) was the most efficient of the three IFNs in conferring resistance PRV infection\; 100 U/ml PoIFN-β inhibited PRV plaque formation 5.3-fold. Compared with PoIFN-β, porcine IFN-γ (PoIFN-γ) was less capable of inhibiting PRV plaque formation (3.3-fold inhibition). Porcine IFN-α (PoIFN-α) had the least capability of the three PoIFNs, and inhibited PRV plaque formation only 1.26-fold. The inhibitory capacity increased to only 2.3-fold with a treatment of 12,800 U/ml PoIFN-α. A combination of PoIFN-γ and PoIFN-α or PoIFN-β inhibited PRV plaque formation 12.8-fold or 100-fold, respectively. Treatment of IBRS-2 cells with PoIFN-α/β and PoIFN-γ inhibited PRV replication 29- or 146-fold. Additionally, real-time PCR analyses of the PRV immediate early (IE) gene revealed that IE mRNA expression was profoundly decreased in cells stimulated with PoIFN-α/β and PoIFN-γ (23.8–133.0-fold) compared with vehicle-treated cells. All the findings indicate that PoIFN-γ acts synergistically with other PoIFNs (PoIFN-α and -β) to potently inhibit PRV replication in vitro. |
Keywords : pseudorabies virus (PRV), porcine interferon-alpha (PoIFN-α), porcine interferon-beta (PoIFN-β), porcine interferon-gamma (PoIFN-γ), immediate-early (IE) gene |
|
