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 Printable version |
Interleukin-18 stimulates HIV-1 replication in a T-cell line. |
European Cytokine Network. Volume 11, Number 1, 47-52, March 2000, Articles originaux
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 Free Article
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Author(s) : S.A. Klein, C. Klebba, D. Kauschat, M. Pape, L. Ozmen, D. Hoelzer, O.G. Ottmann, U. Kalina |
Summary : Interleukin-18 (IL-18) is a recently identified proinflammatory cytokine. Its ability to induce interferon-g suggests a potential virustatic effect. On the other hand, it stimulates NFkB – an activator of HIV replication. Recently, stimulation of HIV-1 in monocytic cells has been demonstrated. In the present study, the influence of IL-18 on HIV-1 replication in lymphatic cells was investigated. Hut78 cells were infected with HIV-1 in the presence of recombinant human IL-18 expressed either in E. coli or eucaryotically by baculovirus in Sf9 cells. HIV-1 replication was monitored by p24 ELISA and endpoint titration of culture supernatants on C8166 cells. The addition of IL-18 led to a 3- to 15-fold enhancement of HIV replication in Hut78 cells. By addition of neutralising monoclonal anti-IL-18 antibodies, this effect of IL-18 was reduced by 75%. Exposure of Hut78 to IL-18 prior to HIV infection could exclude the possibility that IL-18 promotes infection of cells. Taken together, these data provide direct evidence for an IL-18-mediated enhancement of HIV-1 replication in lymphatic cells. |
Keywords : AIDS, interleukin-18, interferon, IGIF, anti IL-18 antibodies, baculovirus. |
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