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HASH(0xb3697b0) |
European Cytokine Network. Volume 13, Number 2, 230-3, June 2002, Articles originaux
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 Free Article
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Author(s) : M. Heesen, B. Bloemeke, B. Bachmann-Mennenga, D. Kunz |
Summary : Genetic variations contribute to the interindividual variance in the cytokine response to endotoxin. The gene of tumor necrosis factor-alpha (TNF-alpha) carries a polymorphism at position -308 of the promoter, consisting of a G/A exchange. To further elucidate the inherited mechanisms influencing cytokine levels, healthy human blood donors were studied. Genotyping for the TNF-alpha -308 and the CD14 -260 C/T promoter polymorphisms was carried out by real-time polymerase chain reaction assay using specific fluorescence-labelled hybridisation probes. A human whole blood assay was used to study the leukocyte TNF-alpha and IL-1beta synthesis capacity upon endotoxin stimulation. We found a linkage disequilibrium between the TNF-alpha -308 G/A and the CD14 -260 C/T polymorphisms (p = 0.043). The CD14 -260 polymorphism was associated with IL-1beta levels (p = 0.033) and higher values were found in C homozygotes. No association was found between the CD14 -260 genotypes or the TNF-alpha -308 - CD14 -260 genotypes and the TNF-alpha response. |
Keywords : CD14, tumor necrosis factor-alpha, interleukin-1beta, endotoxin, genetic polymorphism, linkage disequilibrium, polymerase chain reaction. |
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