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 Printable version |
Involvement of interleukin-6 in the biology and metastatic activity of B16F10 melanoma cells |
European Cytokine Network. Volume 9, Number 2, 187-92, June 1998, Articles originaux
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 Free Article
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Author(s) : A. Garcia de Galdeano, D. Boyano, I. Smith-Zubiaga, A. Alvarez, I. Canton, L. Canavate |
Summary : In previous studies, we demonstrated the presence of the interleukin-2 (IL-2) signalling system in B16F10 murine melanoma and observed that in vitro treatment of B16F10 cells with IL-2, enhanced metastasis. To further understand the role played by interleukins in melanoma, we examined the effect of IL-6 on the metastatic activity and properties of B16 melanoma cells. We observed that B16F10 cells, cultured in the presence of IL-6, showed a clear increase in their metastatic ability, both in the liver and in the lungs. Neither cell proliferation nor in vitro colony formation were affected by IL-6; however, the expression of CD44 and VLA-4 increased. The IL-6 gene was expressed in B16F10 cells as shown by RT-PCR. A slight induction of IL-6 mRNA expression by IL-2 was observed, but not after treatment with IL-1 or IL-6. Nevertheless, no soluble IL-6 could be detected in cell supernatants even after treatment with IL-2. Finally, we tested the effect of IL-1 , IL-6 and IL-2 on the expression of the IL-2 receptor (IL-2R). IL-1 turned out to be the strongest inducer of IL-2R expression in B16F10 cells. Altogether, these data confirm the involvement of interleukins in the biology and metastatic activity of melanoma. |
Keywords : IL-6, metastasis, B16F10 melanoma. |
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