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Apolipoprotein(a) isoforms immunoblotting detection: comparative study of two methods |
Annales de Biologie Clinique. Volume 70, Number 1, 13-7, Janvier-Février 2012, Article original
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Author(s) : Angèle Edjème-Aké, Roselyne Garnotel, Sandrine Vallé-Polneau, Hugues Ahiboh, Marie Laure Hauhouot-Attoungbré, Dagui Monnet, Philippe Gillery |
Summary : This study reports the comparison between two methods (chemiluminescence and enzymatic colorimetry) for revelation of apolipoprotein(a) [apo(a)] isoforms by immunoblotting in 102 Ivorian healthy subjects. Apo(a) isoform sizes were determined by sodium dodecyl sulfate-agarose-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting using enzymatic colorimetry or chemiluminescence. Within-run precision was comprised between 4.9% and 9.2% for colorimetry and between 2.9% and 4.6% for chemiluminescence. Both methods have detected apo(a) isoforms in all patients, even when lipoprotein(a) concentrations were under detection limit (0.02 g/L). The two methods were significantly correlated (r \= 0.96 to 0.98, p<\;0.0001). Even though the chemiluminescence method exhibited better performances than the colorimetric method, both techniques could be used indifferently. |
Keywords : apo(a) isoforms, phenotypying, chemiluminescence, enzymatic colorimetry, healthy subjects, Ivory Coast |
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