Author(s) : J. Taïeb, C. Sarnel, C. Benattar, A. Lindenbaum, Service de biochimie
et d’hormonologie,
Hôpital Antoine-Béclère,
157, rue de la Porte-de-Trivaux,
92141 Clamart cedex.
Summary : We studied the performances of the 17b-estradiol determination with the Kryptor System (Cis Bio international), in assisted medical procreation cycles. The detection is based on Trace® technology. We compared this method with Estradiol-6® method on ACS-180 (Bayer Diagnostics). The Kryptor method is sensitive, has a good precision and accuracy. Comparison with ACS-180 showed a good correlation but results were much higher. Patients under stimulation for assisted procreation were followed by the two methods. The results were close to those obtained with ACS-180 but significantly higher. Thus it was necessary to define the levels which have to be reached before ovulation induction.
Figure 1. A) Épreuve
de dilution d'un sérum de concentration à 8 000 pmol/l par
un pool de sérums d'enfants. B) Épreuve de surcharge d'un
pool de sérums d'enfants par un sérum de concentration à
5 500 pmol/l.
Figure 2. Comparaison
des techniques de dosage du 17beta-estradiol Kryptor® et
ACS-180 : A) régression linéaire. B) méthode des différences.
Figure 3. Exemples de stimulations
sous agoniste du GnRH : A) Cycle sous agoniste du GnRH (Décapeptyl®)
stimulé par HMG (Humegon®) : protocole long. B) Cycle
sous agoniste du GnRH (Lucrin®) stimulé par FSH rec.
(Gonal F®) : protocole long. C) Cycle sous agoniste du GnRH
(Enanthone®) stimulé par FSH rec. (Gonal F®)
: protocole long. D) Cycle sous agoniste du GnRH (Decapeptyl®)
stimulé par HMG (Humegon®) : protocole court.
Figure 4. Exemples de
stimulations simples : A) Cycle spontané. B) Monitorage de l'ovulation
: cycle stimulé par FSH rec. (Puregon®). C) Replacement
d'embryon « cryopréservé » : patiente traitée
par Progynova® et Utrogestan®.
Tableau 1. Performances
de la trousse Estradiol Kryptor®
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