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 Printable version |
Antibody avidity: use for the diagnosis of HIV early infection |
Annales de Biologie Clinique. Volume 59, Number 1, 41-7, Janvier - Février 2001, Articles originaux
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 Résumé
Article gratuit
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Author(s) : H. Le Guillou, A. Le Meur, S. Bourdon, M. Riou, J. Loison, P. Fialaire, J.-M. Chennebault, S. Kouyoumdjian, C. Payan |
Summary : Determination of IgG avidity is useful to distinguish primary infection from reactivation or reinfection in viral, parasitic or bacterial infections. For diagnosis of HIV type 1 primary infection, the detection of IgM antibodies is often useless since they are also found in chronic infection. The usual serology (Elisa, western-blot, p24 antigen) may present no interest if done too late (more than 2 or 3 months after infection). Therefore, we have developed a test to determine the avidity of anti-HIV1 antibodies, using 1 M guanidine as denaturing agent. We have adapted the measurement of avidity to the Axsym automatic system for a routine use. Indeed, since requests for avidity determinations are sporadic, the use of microplates is not convenient. Using this assay, we found a low avidity (less than 50%) in immunocompetent and recent infected patients (less than 6 months), compared to old infected patients (more than 12 months) who had high avidity (80 to 100%). However, early treated patients (in the 6 months after contamination) had also low avidities but with a slower development of antibody maturation (8 to 27 months versus 2 to 8 months in non treated patients). To conclude, the determination of the anti-HIV1 avidity, according to the proper procedures explained here (notion of treatment and/or serious immunodepression), may help the physician to date the infection in each new infected patient who might benefit from an early treatment. |
Keywords : Avidity Maturation Automate HIV1. |
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