Author(s) : M Parmentier, C Al Sayed Mahmoud, M Linder, J Fanni , Nancy-Université, INPL-ENSAIA, Laboratoire de Science et Génie Alimentaires, Vandœuvre-les-Nancy, France.
Summary : The n-3 fatty acids are unanimously considered as high nutritional value molecules, especially Long-Chains PUFA from marine origin. However, most of the products available in the market contain LC-PUFA esterified on the glycerol under the common form of triacylglycerols. Another interesting way can be to esterify the PUFA on polar lipids and especially on Phospholipids. An original patented enzymatic process carried out under mild conditions (low temperature, no use of organic solvent) is presented to produce at industrial scale a Phospho-Lipo-Peptidic Complex, that is particularly rich in DHA esterified on the sn-2 position on PL.
Figure 2 PLPCω3-PUFA bioavailability: preliminary
results on Caco-2 cells.
Figure 3 Extraction of phospholipids from fish roe
(Leigh et al., 2004).
Figure 4 Extraction of phospholipids from
Krill : successive acetone and alcohol treatments (Fotini
Sampalis, 2004).
Figure 5 Marine lipid enzymatic extraction (low
temperature, no use of organic solvent (Linder et al., 2002).
Figure 6 Fatty acid profile of the PL-phase of the
phospho-lipo-peptidic complex.
Figure 7 PLPC Lipid classes by TLC-FID.A:
1st developing tank: hexane/diethyl ether/formic acid
(80:20:0.2, v/v/v) to separate NL after 30 min.B:
2nd developing tank: chloroform/methanol/ammonia
(130:70:10, v/v/v) to separate PL after 40 min.
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