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 Printable version |
Molecular diagnosis of mildew (Plasmopara halstedii) infection in sunflower seeds |
Oléagineux, Corps Gras, Lipides. Volume 8, Number 3, 258-60, Mai - Juin 2001, Fondamental
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 Résumé
Article gratuit
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Author(s) : Véronique SAYS-LESAGE, Cipta MELIALA, Paul NICOLAS, Patricia ROECKEL-DREVET, Denis TOURVIEILLE DE LABROUHE |
Summary : Sunflower downy mildew, caused by the fungus Plasmopara halstedii, is a potentially devastating disease with important economical implications. Because of the risk of propagation of the disease by infected seeds and of the regular observation of metalaxyl-resistant isolates of the fungus, there is a greater need for efficient method to detect the fungus in seed or plant tissue. Therefore, we develop-ed a molecular test to show the presence of sunflower downy mildew in seed samples. Several extraction methods were compared to improve quality and yield of the scanty DNA of the fungus. Both whole and hulled seeds of contaminated samples were analysed by PCR, using Plasmopara halstedii specific primers. We demonstrated that DNA of the pathogen is always detected, notably in the shell fraction. These data are important for the development of a diagnostic kit. |
Keywords : diagnostic, downy mildew, sunflower, Plasmopara halstedii, PCR. |
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