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The search for genes involved in the defence mechanisms of the sunflower, Helianthus annuus L., against the mildew agent Plasmopara halstedii


Oléagineux, Corps Gras, Lipides. Volume 6, Number 4, 337-42, Juillet - Août 1999, Fondamental

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Author(s) : Florence MAZEYRAT , Saïd MOUZEYAR, Denis TOURVIEILLE de LABROUHE, Gérard LEDOIGT

Summary : Identification of genes involved in sunflower defense mechanisms against downy mildew, Plasmopara halstedii, was undertaken in hypocotyls, 24 and 48 hours after secondary infection. Molecular characterization of disease interaction was carried out by studies of the expression of genes known to be involved in plants defense mechanisms such as Phenylalanine ammonia-lyase (PAL), chitinase and ubiquitine. The expression of these genes are induced during incompatible combinations (resistance). Similarly, the use of differential display reverse transcription PCR allowed us to detect 2 cDNA involved in defense mechanisms of sunflower against downy mildew, specifically in resistance mechanisms.

Keywords : Helianthus annuus, downy mildew, Plasmopara halstedii, genes, defense, resistance.

 

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