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 Printable version |
Search for lipid markers evidencing the absence of central nervous tissues in raw materials of bone-source gelatine |
Oléagineux, Corps Gras, Lipides. Volume 9, Number 5, 369-74, Septembre - Octobre 2002, Fondamental
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 Résumé
Article gratuit
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Author(s) : Nicole COMBE, Corinne MIGNEROT, Claude RIDOUX, Sylviane GUEDJ, Christine POISSON |
Summary : Raw materials for gelatine production are obtained through processing bovine bone materials from which fat has been previously removed. These ones are named "heavy bones" and "light bones". Use any raw materials exhibiting a health risk in that which concerns BSE or bovine spongiform encephalopathy (head and skull bones, brains, eyes, tonsils and spinal cord) is forbidden. The aim of this study was to identify one or several lipid characteristics sufficiently specific to central nervous system tissues, allowing them to be used as markers for the latter, in the residual fatty matters of raw materials, for quality tests. Comparison of lipid compositions between bovine Spinal Cord (SC) and bone materials (marrows and tissues of long and flat bones of oxen or pigs, vertebrae) for gelatine production provided three parameters, namely proportions of phospholipids (PL), very long chain fatty acids (VLCFA: 20:0 + 20:1 n-9 and n-7 + 22:0 + 22:1 n-9 + 23:0 + 24:0 + 24:1 n-9) and fatty alcohols analysed as dimethyl acetal (DMA) derivatives. The pertinence of a given parameter (p) for detecting nervous tissues in raw materials was assessed through a factor: Fp = [p]SC /[p]bone materials. The higher is the value of Fp , the greater is the pertinence of the parameter. In regard to these raw materials ("light" and "heavy" bones), the values of FPL ranged from 8 to 15; those of FVLCFA ranged from 25 to 41; that of FDMA was 26 in regard to "light bones", while it reached to infinity in regard to "heavy bones" in which DMA were undetected, therefore this parameter was the most appropriate for detecting nervous system-origin tissues in this raw material used for gelatine production. |
Keywords : gelatine, bones, bovine spinal cord, lipid markers. |
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